The Ukraine crisis is a crisis of state-building，resulted from the failed political and economic transformation and the accompanied ethnic and religious cleavages，and the external intervention has exacerbated the crisis. The European Union introduced corresponding policies at different stages of the crisis，with an attempt to draw Ukraine over to its side. In response to the crisis，the European Union laid stresses on employing soft power and prioritizing guiding public opinion，strengthening its assistance to Ukraine while pushing forward sanctions against Russia，and relying on the international institutions and multilateral frameworks to deal with the conflicts. It is evident that the EU member states diverge greatly on whether to impose sanctions against Russia，while Germany plays a critical role in deciding the contents and methods of the EU’s sanctions. The Ukraine crisis has made profound impacts on the EU，which would lead to an adjustment of the Eastern Partnership and a transformation of the security order in Europe. At last，both the transatlantic and EU-Russia relations have been subject to different degrees of influences.