Since the opening of sixteenth century，the Portuguese had brought a large quantity of people，whom were called Negro by them，from Southeast of Africa，coastal areas of Persian Gulf，Southeast of India and Southeast Asia to Macao and southeast coast of China. These man or women played different roles in this expansion. They may be mercenaries in the war，sailors in the ships，servants in Portuguese families，and someone who could speak Chinese and Portuguese became translators. For their huge number and complicated nationality，the Negro brought to the east had composed a peculiar picture in the history of Sino-West relation which draws much attention of scholars. On the basis of analyzing historical materials，in Chinese，Portuguese and other languages，activities of these Negro in Macao and southeast coast of China would be discussed in this paper，which was ignored before. And this may be helpful to complete the whole expansion-picture of Portuguese in our mind.